1BR PBRM1 KO3
Availability: 3-4 weeks
Organism: Human
Tissue: skin
Model: Genetically modified ; immortalised non-cancer cells
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jessica Downs, Hugang Feng
Institute: The Institute of Cancer Research
Primary Citation: Feng et. al. 2022 Genes Dev 36:790-806 PMID: 35902118
Tool Details
*FOR RESEARCH USE ONLY
- Name: 1BR PBRM1 KO3
- Parental cell: 1BR3-hTERT
- Clone: 3
- Organism: Human
- Gender: Male
- Tissue: skin
- Morphology: Fibroblast
- Growth properties: Adherent
- Model: Genetically modified ; immortalised non-cancer cells
- Model description: PBRM1 knockout
- Crispr: Yes
- Conditional: No
- Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
- Description: 1BR3-hTERT cell line knockout for PBRM1 (clone 3). This cell line has been used for genotypic screening of cancer to predict response to immunotherapies. Specifically to identify functional proteins involved in chromatin re-modelling and how their loss-of-function can regulate DNA damage-induced inflammatory signalling and sensitivity to T-cell-mediated cell killing.
- Application: Chromatin remodelling biology studies
- Biosafety level: 1
Applications
- Application: Chromatin remodelling biology studies
Handling
- Format: Frozen
- Growth medium: DMEM + 10S + 1% Penicillin/Streptomycin
- Temperature: 37C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Cultured in antibiotics: 1% Penicillin/Streptomycin
- Str profiling: See Cellosaurus profile of parental cell line
References
- Feng et. al. 2022 Genes Dev 36:790-806 PMID: 35902118
