#151548

Anti-SNAIL1 [20C8]

Cat. #151548

Anti-SNAIL1 [20C8]

Cat. #: 151548

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: SNAIL1

Class: Monoclonal

Application: WB ; ChIP ; ELISA ; IHC ; IF ; IP ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Melanie Hardman

Institute: Cancer Research Technology

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-SNAIL1 [20C8]
  • Cancer: Breast cancer;Genitourinary cancer;Gynaecologic cancer
  • Cancers detailed: Breast cancer;Broadly Applicable;Epithelial;Ovarian;Prostate
  • Research fields: Cancer;Genetics;Tissue-specific biology
  • Clone: 20C8
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: WB ; ChIP ; ELISA ; IHC ; IF ; IP ; WB
  • Description: The Snail superfamily are zinc finger transcription factors. Overexpression of Snail1 can lead to cells undergoing EMT (epithelial to mesenchymal transition). This is achieved as Snail1 represses expression of E-cadherin, desmoplakin, muc-1, cytokeratin18 while enhances expression of vimentin and fibronectin. Snail1 therefore has a strong association with metastatic disease.
  • Immunogen: Human Snail 1 recombinant protein
  • Isotype: IgG2a kappa
  • Recommended controls: MDAMB-453; MDAMB-435; MDAMB-231; A375

Target Details

  • Target: SNAIL1
  • Tissue cell line specificity: MDAMB-453; MDAMB-435; MDAMB-231; A375
  • Target background: The Snail superfamily are zinc finger transcription factors. Overexpression of Snail1 can lead to cells undergoing EMT (epithelial to mesenchymal transition). This is achieved as Snail1 represses expression of E-cadherin, desmoplakin, muc-1, cytokeratin18 while enhances expression of vimentin and fibronectin. Snail1 therefore has a strong association with metastatic disease.

Applications

  • Application: WB ; ChIP ; ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Yang et al. 2018. Exp Mol Med. 50(1):e420. PMID: 29303507.
  • Goldman et al. 2016. Stem Cell Res. 17(1):62-8. PMID: 27240252.
  • Ju et al. 2015. Cell Death Dis. 6:e1801. PMID: 26136074.
  • Tuhkanen et al. 2009. BMC Cancer. 9:289. PMID: 19695091.
  • Nuclear expression of Snail1 in borderline and malignant epithelial ovarian tumours is associated with tumour progression.
  • Vincent et al. 2009. Nat Cell Biol. 11(8):943-50. PMID: 19597490.
  • A SNAIL1-SMAD3/4 transcriptional repressor complex promotes TGF-beta mediated epithelial-mesenchymal transition.
  • Franc et al. 2006. Oncogene. 25(37):5134-44. PMID: 16568079.
  • Expression of Snail protein in tumor-stroma interface.