#151851

Anti-RSV Subgroup B Phosphoprotein [4-14]

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Cat. #151851

Anti-RSV Subgroup B Phosphoprotein [4-14]

Cat. #: 151851

Unit size: 100 ug

Availability: 1-2 weeks

Target: Human Respiratory Syncytial (RS) virus phosphoprotein VP32

Class: Monoclonal

Application: ELISA ; IF ; WB

Reactivity: Virus

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ayham Alnabulsi

Institute: Vertebrate Antibodies Limited

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-RSV Subgroup B Phosphoprotein [4-14]
  • Research fields: Microbiology
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Strain: Balb/c
  • Reactivity: Virus
  • Host: Mouse
  • Application: ELISA ; IF ; WB
  • Description: Immunoblot assays performed in parallel using as antigen 19 clinical RS virus isolates of subgroups A and B (isolated between 1972 and 1984) showed that this antibody reacted only with the phosphoprotein of RS virus subgroup B (Gimenez et al, 1986). RS virus subgroups A and B circulate concurrently in the human population. Infections with RS virus subgroup A are thought to produce more severe disease than infections with RS virus subgroup B. Consequently, it is beneficial for patient management to know early during an RS virus infection whether the patient is infected with RS virus subgroup A or B. Thus, this antibody is relevant for as a diagnostic tool to determine, soon after infection, the specific RS virus subgroup responsible for the RS virus infection. A diagnostic kit protocol is described in Notes.
  • Immunogen: The immunogen was gradient-purified RSN-2 virus (subgroup B) that was then treated with 0.1% SDS at 100°C for 2 min. The procedure used to produce this antibody is described in Gimenez et al. (1984).
  • Isotype: IgM
  • Myeloma used: P3X63Ag8.653
  • Recommended controls: Immunoblot: Partial purified PVM (lane 2). Partial purified BRS virus (lane 3). Gradient-purified RSN-2 virus (lane1 & 4): 5ug/lane. First antibody: 4-14 antibody (lanes 1,2,3 & 4). Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells

Target Details

  • Target: Human Respiratory Syncytial (RS) virus phosphoprotein VP32
  • Target background: Immunoblot assays performed in parallel using as antigen 19 clinical RS virus isolates of subgroups A and B (isolated between 1972 and 1984) showed that this antibody reacted only with the phosphoprotein of RS virus subgroup B (Gimenez et al, 1986). RS virus subgroups A and B circulate concurrently in the human population. Infections with RS virus subgroup A are thought to produce more severe disease than infections with RS virus subgroup B. Consequently, it is beneficial for patient management to know early during an RS virus infection whether the patient is infected with RS virus subgroup A or B. Thus, this antibody is relevant for as a diagnostic tool to determine, soon after infection, the specific RS virus subgroup responsible for the RS virus infection. A diagnostic kit protocol is described in Notes.

Applications

  • Application: ELISA ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1mg/ml
  • Unit size: 100 ug
  • Storage buffer: Dulbecco’s media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Gimenez et al. 1986. Journal General Virology, 67: 863-70. PMID: 3517224 Gimenez et al. 1984. Journal General Virology, 65: 963-71. PMID: 6202832 Gimenez et al. 1987. Journal General Virology, 68: 1267-75. PMID: 3572364