![Western blotting was performed on overexpression human lysates using anti-PALB2 [M41-P3F3] applied as tissue culture supernatant.](https://cancertools.org/wp-content/uploads/b92f0d79-323a-4a98-9ad6-1b77ccfd8cca.jpg)
Cat. #152698
Anti-PALB2 [M41-P3F3]
Cat. #: 152698
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Partner and localizer of BRCA2
Class: Monoclonal
Application: ELISA ; IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ayham Alnabulsi
Institute: Vertebrate Antibodies Limited
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-PALB2 [M41-P3F3]
- Cancer: Breast cancer;Gynaecologic cancer
- Cancers detailed: Breast cancer;Broadly Applicable;Ovarian
- Research fields: Cancer;Genetics
- Clone: M41-P3F3
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Disease: Breast Cancer
- Application: ELISA ; IHC ; WB
- Description: PALB2 plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures.
- Immunogen: Peptide Sequence KENQFLMPPEE
- Isotype: IgG1 kappa
- Recommended controls: IHC- formalin-fixed, paraffin-embedded multi tumour tissue microarray
Target Details
- Target: Partner and localizer of BRCA2
- Tissue cell line specificity: IHC- formalin-fixed, paraffin-embedded multi tumour tissue microarray
- Target background: PALB2 plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Fnly cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures.
Applications
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
