Cat. #151152
Anti-Myofibroblast marker [PR 2D3]
Cat. #: 151152
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Myofibroblast marker
Class: Monoclonal
Application: IP ; WB ; IHC ; IF
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Myofibroblast marker [PR 2D3]
- Alternate name: Proliferating Cell Nuclear Antigen; DNA Polymerase Delta Auxiliary Protein; ATLD2
- Research fields: Cell biology
- Clone: PR 2D3
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 140 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IP ; WB ; IHC ; IF
- Description: PR 2D3 reacts with a cell membrane component of cells in the pericrypt sheath; with smooth muscle cells and myofibroblasts. This antibody is considered to be the gold standard for the identification of myofibroblasts.
- Immunogen: Crude homogenate of normal colorectal mucosa.
- Isotype: IgG1
- Myeloma used: NS0
Target Details
- Target: Myofibroblast marker
- Molecular weight: 140 kDa
- Target background: PR 2D3 reacts with a cell membrane component of cells in the pericrypt sheath; with smooth muscle cells and myofibroblasts. This antibody is considered to be the gold standard for the identification of myofibroblasts.
Applications
- Application: IP ; WB ; IHC ; IF
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Hsia et al. 2016. Proc Natl Acad Sci U S A. :. PMID: 27036009.
- Myofibroblasts are distinguished from activated skin fibroblasts by the expression of AOC3 and other associated markers.
- Kirkland et al. 2001. J Cell Sci. 114(Pt 11):2055-64. PMID: 11493641.
- Collagen IV synthesis is restricted to the enteroendocrine pathway during multilineage differentiation of human colorectal epithelial stem cells.
- Richman et al. 1987. J Clin Pathol. 40(6):593-600. PMID: 3301906.
- Colonic pericrypt sheath cells: characterisation of cell type with new monoclonal antibody.
