Inhibition of T lymphocyte or cell adhesion to fibronectin was most efficient when the inhibitory antibodies to ?4/?1 and ?5/?1 were used together. This antibody works well with formalin, 4 % paraformadehyde, acetone-methanol or ice-cold methanol fixation.
| Inventor | Institute |
|---|---|
| Elizabeth A. Wayner, William Carter | Fred Hutchinson Cancer Center |
| Cat. #: | 161661 |
|---|---|
| Tool sub type: | Primary antibody |
| Research Fields: | Cell biology |
| Application: | FFPE, Function Blocking, Immunoprecipitation, Western Blot |
| Target: | Integrin alpha-5 (CD49e) |
| Reactivity: | Human |
| Clone: | P1D6 |
| Host: | Mouse |
| Class: | Monoclonal |
| Primary citation: | Wayner et al. 1988. Journal of Cell Biology.107(5):1881-91. PMID: 2846588 |
| Product description: | MoAb P1D6 was developed to identify a new fibronectin receptor that is identical to the integrin receptor ?5/?1. P1D6 neutralizes binding of the ?5/?1 receptor to the cell adhesion domain of fibronectin only. The antibody does not inhibit binding to collagen, laminin or vitronectin. P1D6 recognizes native protein only. |
|---|---|
| Isotype: | IgG1 |
| Molecular weight: | 115 kDa |
| Immunogen: | non-trypsinized WI-38 VA13 or HT-1080 cells |
| Target background: | ?5?1 complex of Collagen receptors: I, II, III, IV. |
|---|
| Storage conditions: | For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing. |
|---|---|
| Shipping conditions: | Dry ice |
| References: |
Wayner et al. 1988. Journal of Cell Biology.107(5):1881-91.PMID: 2846588 Mould et al.1997.J Biol CHem.272(28):17283-92.PMID: 9211865 Wayner.2007. Methods Enzymol.426:117-53.PMID: 17697883 Wayner et al.1989. Journal of Cell Biology. 109(3):1321-30 Carter et al.1990. J Cell Biol.111(6 Pt 2):3141-54.PMID: 2269668 Carter et al.1990. J Cell Biol.111(6):3141-54.PMID: 2269668 Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064 |
|---|
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