Anti-integrin a2 [P1E6]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-integrin a2 [P1E6]
• Research fields: Cell biology
• Clone: P1E6
• Tool sub type: Primary antibody
• Class: Monoclonal
• Molecular weight: Predicted: 129 kDa; Apparent: 150 kDa
• Strain: Application Notes: For Western Blot, immunoblotting works under non-reducing conditions ; Immunostaining with frozen or acetone fixation (not formalin) ; Doesn't work for FFPE.
• Reactivity: Human
• Host: Mouse
• Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Description: Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. Integrin ?2 (P1E6) is a mouse monoclonal antibody raised against HT-1080. Antibodies were developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. This antibody was found to Inhibit binding to collagens recpetors I, III, IV and VI.
• Immunogen: HT1080 human fibrosarcoma cell line
• Isotype: IgG1

Target Details

• Target: Integrin alpha 2 (CD49b)
• Molecular weight: Predicted: 129 kDa; Apparent: 150 kDa
• Target background: ?2?1 complex of Collagen receptors: I, II, III, IV

Applications

• Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

• Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

References

• Wayner et al. 1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727
• Kamata et al.1994.J Biol Chem. 269(13):9659-63.PMID: 7511592
• Tuckwell et al. 1995.J Cell Sci. 108 ( Pt 4):1629-37.PMID: 7615681