#151166

Anti-HPV16 E1/E4 [TVG 403]

Cat. #151166

Anti-HPV16 E1/E4 [TVG 403]

Cat. #: 151166

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: HPV-16 E1/E4 (early proteins E1 and E4).

Class: Monoclonal

Application: ELISA ; FACS ; IHC

Reactivity: Human papilloma virus

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Peter Beverley

Institute: University of Cambridge

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-HPV16 E1/E4 [TVG 403]
  • Alternate name: Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; ELAM-1 Ligand Fucosyltransferase; FUC-TIV; FCT3A; ELFT; Stage-Specific Embryonic Antigen; Alpha (1,3) Fucosyltransferase; EC 2.4.1.65; Lewis X; SSEA-1; FUTIV; CD15; LeX
  • Cancer: Gynaecologic cancer
  • Cancers detailed: Cervical
  • Research fields: Cancer;Microbiology
  • Clone: TVG 403
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 10 kDa
  • Strain: CBA
  • Reactivity: Human papilloma virus
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC
  • Description: TVG 402 and TVG 403 were the first antibodies against HPV16 E1 & E4 and should complement those already available to E7 and L1 for the screening of frozen sections of clinical biopsies. They will be of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.
  • Immunogen: Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide.
  • Isotype: IgG1
  • Myeloma used: NS0

Target Details

  • Target: HPV-16 E1/E4 (early proteins E1 and E4).
  • Molecular weight: 10 kDa
  • Target background: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer case. E1 and E2 are proteins involved in the regulation of viral DNA replication.

Applications

  • Application: ELISA ; FACS ; IHC

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Doorbar et al. 2000. J Virol. 74(21):10081-95. PMID: 11024137.
  • The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus.
  • Doorbar et al. 1992. Virology. 187(1):353-9. PMID: 1371027.
  • Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein.