![Western blotting on lysates prepared from SARS-CoV-infected Vero E6 cells (+) using anti-S?10 [1A9]. A lysate from mock Vero E6 cells is included as a negative control using anti-S?10 [1A9].](https://cancertools.org/wp-content/uploads/db5906d5-1f70-459d-8046-2fd43db317e8.png "db5906d5-1f70-459d-8046-2fd43db317e8.png")
Cat. #152652
Anti-Delta10 [1A9]
Cat. #: 152652
Unit size: 100 ug
Availability: 3-5 days
Target: S protein of the SARS coronavirus
Class: Monoclonal
Application: FACS ; Fn ; WB
Reactivity: Virus
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: A*STAR Accelerate Technologies Pte Ltd
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Delta10 [1A9]
- Research fields: Microbiology
- Clone: 1A9
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Virus
- Host: Mouse
- Application: FACS ; Fn ; WB
- Description: Severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. Both of these processes are critical for virus entry into host cells. The SÄÂĂÂ10 is a fragment containing residues from 1029 to 1129 of the S protein, which is a domain that is crucial for the fusion process.
- Immunogen: GST-S?10 fusion protein
- Isotype: IgG1 kappa
- Myeloma used: Sp2/0-Ag14
- Recommended controls: SARS-CoV-infected Vero E6 cells
Target Details
- Target: S protein of the SARS coronavirus
- Target background: Severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes for four structural proteins, namely nucleocaspid (N), spike (S), membrane (M) and envelope (E). The S protein, which forms morphologically characteristic projections on the virion surface, is responsible for binding to cellular receptor and mediating the fusion between viral and cellular membranes. Both of these processes are critical for virus entry into host cells. The SÄÂĂÂ10 is a fragment containing residues from 1029 to 1129 of the S protein, which is a domain that is crucial for the fusion process.
Applications
- Application: FACS ; Fn ; WB
Handling
- Format: Liquid
- Concentration: 1mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Ng et al. 2014. PLoS One. 9(7):e102415. PMID: 25019613.
- Substitution at aspartic acid 1128 in the SARS coronavirus spike glycoprotein mediates escape from a S2 domain-targeting neutralizing monoclonal antibody.
- Lip et al. 2006. J Virol. 80(2):941-50. PMID: 16378996.
- Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus.
