#151088

Anti-CyclinA [E23.1]

Cat. #151088

Anti-CyclinA [E23.1]

Cat. #: 151088

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Cyclin A

Class: Monoclonal

Application: FACS ; IHC ; IF ; IP ; WB

Reactivity: Mammalian

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Julian Gannon

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CyclinA [E23.1]
  • Alternate name: CCNA1; Cyclin A1; Testicular Tissue Protein Li 34; CT146
  • Research fields: Cancer;Cell biology;Genetics
  • Clone: E23.1
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Strain: Balb/c
  • Reactivity: Mammalian
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: E23.1 is extremely sensitive for immunoblotting Cyclin A.
  • Immunogen: Bovine Cyclin A fusion protein.
  • Immunogen uniprot id: P17927
  • Isotype: IgG2a
  • Myeloma used: Sp2/0-Ag14

Target Details

  • Target: Cyclin A
  • Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase.

Applications

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Hagen et al. 2013. Cell Div. 8(1):10. PMID: 23886499.
  • Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis.
  • Li et al. 2012. Proc Natl Acad Sci U S A. 109(31):12550-5. PMID: 22802651.
  • Gap 1 phase length and mouse embryonic stem cell self-renewal.
  • Hogg et al. 2011. Free Radic Biol Med. 50(9):1065-74. PMID: 21256959.
  • Effect of nitric oxide on neointimal hyperplasia based on sex and hormone status.
  • Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
  • Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
  • Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
  • Simian virus 40 large T antigen associates with cyclin A and p33cdk2.