Cat. #151581
Anti-CLEC2 [17D9]
Cat. #: 151581
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Ctype lectin-like receptor (CLEC2)
Class: Monoclonal
Application: FACS ; WB
Reactivity: Mouse
Host: Rat
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Caetano Reis e Sousa
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CLEC2 [17D9]
- Research fields: Immunology;Tissue-specific biology
- Clone: 17D9
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Mouse
- Host: Rat
- Application: FACS ; WB
- Description: Monoclonal antibody used to study the expression pattern of CLEC-2 receptor.
- Immunogen: RBL-2H3 cells stably expressing HA-tagged mouse CLEC-2
- Immunogen uniprot id: Q9P126
- Isotype: IgG2b
- Myeloma used: Y3.AG.1.2.3
Target Details
- Target: Ctype lectin-like receptor (CLEC2)
- Target background: CLEC-2 belongs to a sub-family of C-type lectin receptors that signal through Syk via a single YxxL motif (hemITAM). CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower level on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. CLEC-2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC-2 also functions as an activation receptor on monocytes and neutrophils to induce phagocytosis and proinflammatory cytokine production. This antibody can be used as a selective agonist of CLEC-2 and also to study its expression pattern.
Applications
- Application: FACS ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-CLEC2 [AYP1]
References
- Kerrigan et al. 2009. J Immunol. 182(7):4150-7. PMID: 19299712.
- CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils.
