#153780

Anti-CEP164 [1F3G10]

Cat. #153780

Anti-CEP164 [1F3G10]

Cat. #: 153780

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 10-12 weeks

Target: Anti-CEP164 [1F3G10]

Class: Monoclonal

Application: IHC ; IF ; IP ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ciaran Morrison

Institute: National University of Ireland, Galway; Centre For Chromosome Biology

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-CEP164 [1F3G10]
  • Alternate name: Centrosomal protein of 164 kDa, KIAA152, NPHP15
  • Clone: [1F3G10]
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 164 kDa (runs c.190)
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IF ; IP ; WB
  • Description: CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.
  • Immunogen: Recombinant human CEP164 amino acids 6-296 (bacterially expressed from pGEX4T as a GST fusion, then thrombin-cleaved and purified).
  • Isotype: IgG1 kappa
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: hTERT-RPE1 Cell Line

Target Details

  • Target: Anti-CEP164 [1F3G10]
  • Molecular weight: 164 kDa (runs c.190)
  • Tissue cell line specificity: hTERT-RPE1 Cell Line
  • Target background: CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.

Applications

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Flynn et al. 2020. Hum Mol Genet. 29(3):407-417. PMID: 31868218.
  • Khouj et al. 2019. J Cell Sci. 132(19):. PMID: 31492759.
  • Ogungbenro et al. 2018. J Cell Biol. 217(4):1205-1215. PMID: 29440264.
  • Sieben et al. 2018. Nat Methods. 15(10):777-780. PMID: 30275574.
  • Daly et al. 2016. J Cell Sci. 129(9):1769-74. PMID: 26966185.