Scientific figure containing flow cytometry histograms

Cat. #162442

Anti-CAR Whitlow Linker [1G2G2]

Cat. #: 162442

Unit size: 1 mg

Target: Chimeric antigen receptor Whitlow scFv peptide linker

Application: Flow cytometry, IHC

Host: Mouse

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

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Contributor

Inventor: Erik Kimble, Jocelyn Wright

Institute: Fred Hutchinson Cancer Center

Primary Citation: Kimble et al. 2025.J Immunother Cancer. 2025 Nov 18;13(11):e013123. PMID: 41253496

Tool Details

Target Details

Applications

Handling

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Name: Anti-CAR Whitlow Linker [1G2G2]
Conjugation: Unconjugated
Host: Mouse
Application: Flow cytometry, IHC
Description: The anti‑CAR Whitlow linker peptide 1G2G2 clone is a murine monoclonal antibody that binds cells expressing Whitlow linker‑containing CARs in vitro. In flow cytometry, 1G2G2 stains engineered T cells expressing FMC63‑ or 1H7‑Whitlow CARs, but not the corresponding FMC63‑ or 1H7‑G4S CARs. However, in binding kinetic studies using recombinant proteins, 1G2G2 showed no observable binding to FMC63‑Whitlow scFv or the Whitlow peptide, and no binding to the G4S4 peptide, under the assay conditions used.
Immunogen: Synthetic Whitlow peptide
Isotype: IgG2a

Target Details

Target: Chimeric antigen receptor Whitlow scFv peptide linker
Target background: While CAR T-cell therapies have revolutionized the treatment of B-lineage malignancies, high-resolution tracking of CAR-engineered cells within the tumor microenvironment (TME) remains a significant technical challenge, particularly in archival formalin-fixed paraffin-embedded (FFPE) tissues. To address this, murine monoclonal antibodies (mAbs) have been developed to specifically target the Whitlow linker, a synthetic peptide commonly utilized in the scFv domains of multiple FDA-approved CAR products (e.g., axi-cel, liso-cel). Because this linker is absent in native human tissue and conserved across various antigen specificities, these mAbs provide a universal tool for the in situ identification, selection, and functional analysis of CAR-expressing cells. This methodology enables a more precise evaluation of CAR T-cell infiltration, persistence, and correlation with clinical outcomes or toxicities.

Applications

Application: Flow cytometry, IHC

Handling

Unit size: 1 mg