Anti-CAR Whitlow Linker [1B3b]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CAR Whitlow Linker [1B3b]
• Clone: 1B3b
• Class: Monoclonal
• Conjugation: Unconjugated
• Host: Mouse
• Application: Flow cytometry, IHC
• Description: The anti-CAR Whitlow linker peptide 1B3b clone has been developed to detect cells expressing Whitlow linker-containing CARs with different antigen specificities, including those harbouring the widely employed anti-CD19 FMC63-derived scFv as well as other scFvs, such as those targeting B-cell maturation antigen (BCMA) or CD33. It has been demonstrated to have a mean KD of 1 to 3.4 µM when tested for binding kinetics against FMC63-Whitlow scFv protein and the Whitlow peptide. No binding to G4S4 peptide was found. In addition, the 1B3b antibody has been demonstrated to stain engineered T cells expressing 1H7- or FMC63-Whiltow, but not 1H7- or FMC63- G4S4 by FACS analysis.
• Immunogen: Synthetic Whitlow peptide
• Isotype: IgG2a

Target Details

• Target: Chimeric antigen receptor Whitlow scFv peptide linker
• Target background: While CAR T-cell therapies have revolutionized the treatment of B-lineage malignancies, high-resolution tracking of CAR-engineered cells within the tumor microenvironment (TME) remains a significant technical challenge, particularly in archival formalin-fixed paraffin-embedded (FFPE) tissues. To address this, murine monoclonal antibodies (mAbs) have been developed to specifically target the Whitlow linker, a synthetic peptide commonly utilized in the scFv domains of multiple FDA-approved CAR products (e.g., axi-cel, liso-cel). Because this linker is absent in native human tissue and conserved across various antigen specificities, these mAbs provide a universal tool for the in situ identification, selection, and functional analysis of CAR-expressing cells. This methodology enables a more precise evaluation of CAR T-cell infiltration, persistence, and correlation with clinical outcomes or toxicities.

Applications

• Application: Flow cytometry, IHC

Handling

• Unit size: 1 mg