Cat. #152712
Anti-5T4 [B5C12]
Cat. #: 152712
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: 5T4 oncofetal glycoprotein
Class: Monoclonal
Application: ELISA ; FACS ; IF ; WB
Reactivity: Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Stern
Institute: Cancer Research UK, Manchester Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-5T4 [B5C12]
- Alternate name: Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
- Research fields: Cancer;Stem cell biology
- Clone: P1C9
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 46.5 kDa
- Strain: C57BL/6
- Reactivity: Mouse
- Host: Mouse
- Application: ELISA ; FACS ; IF ; WB
- Description: The murine 5T4 monoclonal antibody, clone B5C12, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.
- Isotype: IgG2b kappa
Target Details
- Target: 5T4 oncofetal glycoprotein
- Molecular weight: 46.5 kDa
- Target background: The murine 5T4 monoclonal antibody, clone B5C12, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.
Applications
- Application: ELISA ; FACS ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
Related Tools
- Related tools: Anti-5T4 [B1C3] ; Anti-5T4 [B3F1] ; Anti-5T4 [B5C9]
References
- Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.
- CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.
- Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.
- The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.
- Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.
- Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.
