RCC-FG2 + PBRM1 Clone 13
RCC-FG2 cell line deficient for PBRM1 stably expressing DOX-inducible PBRM1 construct (Clone 13)
Contributors
| Inventor | Institute |
|---|---|
| Jessica Downs, Hugang Feng | The Institute of Cancer Research |
| Cat. #: | 161867 |
|---|---|
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | kidney |
| Gender: | Male |
| Model: | Genetically modified |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Product description: | RCC-FG2 cell line deficient for PBRM1 stably expressing DOX-inducible PBRM1 construct (Clone 13). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis. |
|---|---|
| Gender: | Male |
| CRISPR: | Yes |
| Conditional: | Yes |
| Conditional description: | Conditional expression of wild type PBRM1 gene following deoxycycline treatment |
| Cellosaurus ID: | CVCL_5873 |
| Parental cell line: | RCC-FG2 |
| Model description: | Conditional re-expression of wild type PBRM1 gene |
| Products or characteristics of interest: | Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing. |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | RPMI-1640 + 10S + 1% Penicillin/Streptomycin |
| Temperature: | 37C |
| Atmosphere: | 5% CO2 in air |
| Biosafety level: | 1 |
| References: |
Unpublished |
|---|
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