Cat. #151753
Anti-Phospho-cdc20 (Thr79) [BT2.1]
Cat. #: 151753
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: cdc20
Class: Monoclonal
Application: ELISA ; WB
Reactivity: Xenopus laevis
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Phospho-cdc20 (Thr79) [BT2.1]
- Alternate name: APC/C activator protein CDC2; Cell division control protein 2; YGL116W; Cell Division Cycle 2; P55CDC; CDC2 Cell Division Cycle 2 Homolog; Cell Division Cycle 2 Homolog; BA276H19.3; CDC2A
- Research fields: Cell biology
- Clone: BT2.1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 55 kDa
- Reactivity: Xenopus laevis
- Host: Mouse
- Application: ELISA ; WB
- Description: Cdc20 (fizzy protein) is associated with the activation of the anaphase-promoting complex at the onset of anaphase. Cdc20 is phosphorylated during mitosis so this epitope is a good marker of cell cycle progression
- Immunogen: GPKMQGpTPSRAG. pT represents phosphorylated Thr79.
- Isotype: Not Known
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Xenopus egg extract
Target Details
- Target: cdc20
- Molecular weight: 55 kDa
- Tissue cell line specificity: Xenopus egg extract
- Target background: Cdc20 (fizzy protein) is associated with the activation of the anaphase-promoting complex at the onset of anaphase. Cdc20 is phosphorylated during mitosis so this epitope is a good marker of cell cycle progression
Applications
- Application: ELISA ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Labit et al. 2012. EMBO J. 31(15):3351-62. PMID: 22713866.
- Dephosphorylation of Cdc20 is required for its C-box-dependent activation of the APC/C.
