#160861

TetR-controlled recombination-based in vivo expression technology (TRIVET) vector

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Cat. #160861

TetR-controlled recombination-based in vivo expression technology (TRIVET) vector

Cat. #: 160861

Sub-type: Reporter

Availability: Please enquire for quantities and pricing

Target: Cholera

Bacterial Resistance: pTRIVET - ampicillin, tpc reporter cassette - chloramphenicol

Selectable Markers: PhoA activity

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Stefan Schild

Institute: University of Graz

Tool Details
Target Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: TetR-controlled recombination-based in vivo expression technology (TRIVET) vector
  • Alternate name: TRIVET
  • Research fields: Microbiology
  • Tool sub type: Reporter
  • Bacterial resistance: pTRIVET - ampicillin, tpc reporter cassette - chloramphenicol
  • Selectable markers: PhoA activity
  • Description: This system is based upon recombination-based in vivo expression technology (RIVET) and allows the detection of transient gene silencing events even in a subset of a complex, heterogenous bacterial population. It requires all three components; the tpc reporter cassette, pTRIVET and pTRIVET1. It can be used as a random approach to identify in vivo repressed genes during intestinal colonization and as a specific approach to study conditional transcriptional silencing of a gene of interest. The protocol for which can be found at PMID: 33659431.
  • Additional notes: Vibrio cholerae gene expression

Target Details

  • Target: Cholera

Application Details

  • Application notes: This system is based upon recombination-based in vivo expression technology (RIVET) and allows the detection of transient gene silencing events even in a subset of a complex, heterogenous bacterial population. It requires all three components; the tpc reporter cassette, pTRIVET and pTRIVET1. It can be used as a random approach to identify in vivo repressed genes during intestinal colonization and as a specific approach to study conditional transcriptional silencing of a gene of interest. The protocol for which can be found at PMID: 33659431.

References

  • 33659431
  • Cakar et al. 2018. Proc Natl Acad Sci U S A. 115(10):E2376-E2385. PMID: 29463743.