TetR-controlled recombination-based in vivo expression technology (TRIVET) vector

This system is based upon recombination-based in vivo expression technology (RIVET) and allows the detection of transient gene silencing events even in a subset of a complex, heterogenous bacterial population. It requires all three components; the tpc reporter cassette, pTRIVET and pTRIVET1. It can be used as a random approach to identify in vivo repressed genes during […]

Contributors

Inventor Institute
Stefan Schild University of Graz
Cat. #: 160861
Tool sub type: Reporter
Research Fields: Microbiology
Target: Cholera
Bacterial resistance: pTRIVET – ampicillin, tpc reporter cassette – chloramphenicol
Selectable markers: PhoA activity
Alternate name: TRIVET
Product description: This system is based upon recombination-based in vivo expression technology (RIVET) and allows the detection of transient gene silencing events even in a subset of a complex, heterogenous bacterial population. It requires all three components; the tpc reporter cassette, pTRIVET and pTRIVET1. It can be used as a random approach to identify in vivo repressed genes during intestinal colonization and as a specific approach to study conditional transcriptional silencing of a gene of interest. The protocol for which can be found at PMID: 33659431.
Additional notes: Vibrio cholerae gene expression
Application notes: This system is based upon recombination-based in vivo expression technology (RIVET) and allows the detection of transient gene silencing events even in a subset of a complex, heterogenous bacterial population. It requires all three components; the tpc reporter cassette, pTRIVET and pTRIVET1. It can be used as a random approach to identify in vivo repressed genes during intestinal colonization and as a specific approach to study conditional transcriptional silencing of a gene of interest. The protocol for which can be found at PMID: 33659431.
References:

33659431

Cakar et al. 2018. Proc Natl Acad Sci U S A. 115(10):E2376-E2385. PMID: 29463743.

Related Tools for Vectors

Cat. # Tool Name
151911 pGEX 6P1 CDK2 (552) Vector Key Info View Tool
152049 pJEK11 Beta-Synuclein Vector Key Info View Tool
152050 pJEK21 Gamma-Synuclein Vector Key Info View Tool
152742 Chicken IgY precipitating protein Vector Key Info View Tool
153546 pHyg-AID(1-114)-8myc Plasmid Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.