pJEK6 Alpha-Synuclein E46K Vector

pJEK6 (“E46K”) is a derivative of pET15b with the open reading frame encoding the E46K mutant human alpha synuclein (Îą-synuclein) cloned in via the NdeI and BamHI restriction sites. It was constructed via site specific mutagenesis of pJEK1, replacing the G at position 136 in the ORF nucleotide sequence with A, thus altering the 46th […]

Contributors

Inventor Institute
Dr Fiona Benson Lancaster University
Cat. #: 152051
Research Fields: Neurobiology
Product description: pJEK6 ("E46K") is a derivative of pET15b with the open reading frame encoding the E46K mutant human alpha synuclein (Îą-synuclein) cloned in via the NdeI and BamHI restriction sites. It was constructed via site specific mutagenesis of pJEK1, replacing the G at position 136 in the ORF nucleotide sequence with A, thus altering the 46th codon from GAG encoding Glutamic Acid (E) to AAG encoding Lys (K). In this construct E46K Îą-synuclein is expressed as a fusion protein with an N-terminal six His tag.
Additional notes: Alpha synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. Among the familial mutations of α-synuclein in Parkinson′s disease, E46K has the greatest potential to aggregate.
Application notes: pJEK6 ("E46K") is a derivative of pET15b with the open reading frame encoding the E46K mutant human alpha synuclein (α-synuclein) cloned in via the NdeI and BamHI restriction sites. It was constructed via site specific mutagenesis of pJEK1, replacing the G at position 136 in the ORF nucleotide sequence with A, thus altering the 46th codon from GAG encoding Glutamic Acid (E) to AAG encoding Lys (K). In this construct E46K α-synuclein is expressed as a fusion protein with an N-terminal six His tag.

Related Tools for Vectors

Cat. # Tool Name
152049 pJEK11 Beta-Synuclein Vector Key Info View Tool
152052 pKH1 Beta-Synuclein V70M Vector Key Info View Tool
152742 Chicken IgY precipitating protein Vector Key Info View Tool
153287 pCMV-iNOS Vector Key Info View Tool
153538 pSIEW-RUNX1-EVI1 Vector Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.