#153872

pROSA-GUIDE

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Cat. #153872

pROSA-GUIDE

Cat. #: 153872

Sub-type: Mammalian Expression, Lentiviral, CRISPR

Availability: 3-5 days

Target: Gt(ROSA)26Sor; ROSA26

Bacterial Resistance: Ampicillin

Selectable Markers: Puromycin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Dr Andrew Stephens ; Amy Wilson

Institute: Hudson Institute Of Medical Research

Tool Details
Target Details
Application Details

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pROSA-GUIDE
  • Research fields: Cell biology;Genetics
  • Tool sub type: Mammalian Expression, Lentiviral, CRISPR
  • Backbone size without insert: 11530
  • Bacterial resistance: Ampicillin
  • Selectable markers: Puromycin
  • Description: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward
  • Recommended controls: -

Target Details

  • Target: Gt(ROSA)26Sor; ROSA26

Application Details

  • Application notes: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward