HEK293DeltaNDUFS2 Cell Line

A CRISPR/Cas9-edited human HEK293 knockout cell line with disrupted NDUFS2, used to study mitochondrial complex I deficiency, mitochondrial energetics and disease-associated mitochondrial dysfunction.

Contributors

Inventor Institute
Abey Bandara Virginia Tech
SKU: 156497
Product description: HEK293DeltaNDUFS2 is a CRISPR/Cas9-edited human HEK293 knockout cell line with disruption of NDUFS2, a core subunit of mitochondrial complex I. The mutation significantly impairs complex I function and provides a model for studying isolated complex I deficiency, mitochondrial energetics, reactive oxygen species generation, membrane integrity, apoptosis and mitochondrial disease biology. The mutant grows substantially more slowly than parental HEK293 cells, making the parental line an appropriate experimental control.
Alternate name: HEK293?NDUFS2
Production details: The cell line is currently stored at Virginia Tech. By culturing approximately 1 million frozen cells in growth media, approximately 40 million cells can be harvested in a two weeks time.
Additional notes: CRISPR edited HEK293 cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can prov…
Cellosaurus ID: CVCL_C0D2
Parental cell line: HEK293
Disease: Leighs
Related tools: Mitochondrial complex II mutated SDHD cell line
Cat. #: 156497
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy; Drug development; Metabolism
Organism: Human
Model: Knock-Out
Growth properties: The mutant grows much slower than the parent cell line. The doubling time of the mutant is 3 times higher than that of the parent cell line.
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin at 37?‚°C temperature and 5-6% CO2.
Recommended controls: HEK293 parent line
Mycoplasma free: Yes
References:

Bandara et al. 2021. Mitochondrion. 58:160-168. PMID: 33744462.

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