Omega-E Packaging Cell Line

Exclusive Tool

The Omega-E Packaging Cell Line ias an helper-free packaging cell line & shuttle vector system enabling high-titer production of recombinant retroviral vectors for mammalian gene transfer, with significantly reduced probability of replication-competent retrovirus generation.

Contributors

Inventor Institute
Hartmut Land, Jay Morgenstern Cancer Research UK, London Research Institute: Lincoln's Inn Fields
SKU: 151463
Product description: Helper-free packaging cell line developed by Morgenstern and Land in 1990 for safe, high-titre pBABE vector production. Features split gag-pol/env genes that minimise replication competent virus production, while producing consistent viral titers of 10^6-10^7 cfu/ml. Key component of the proven pBABE retroviral system for custom gene expression experiments. Use with any of the pBABE vectors (puro, neo, hygro and zeo) for complete retroviral gene delivery capabilities.
Conditional: Yes
Production details: The Omega E and pBabe recombinant retroviral system utilises shuttle vectors with multiple drug selection markers (the pBabe vector series) for increased flexibility of gene transfer vector design, and a third generation, helper-free packaging cell line (Omega E) derived from mouse NIH 3T3 cell lines, enabling high-titer production of Moloney murine leukemia virus for use in mammalian gene transfer
Cellosaurus ID: CVCL_2151
Parental cell line: NIH/3T3
Cat. #: 151463
Provenance and Ownership: The Omega-E Packaging Cell Line is owned by Cancer Research UK. CancerTools supplies the Omega-E Packaging Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing Omega-E Packaging Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Genetics
Organism: Mouse
Tissue: Embryo
Model: Packaging
Growth properties: Recombinant retroviral production
Format: Frozen
Shipping conditions: Dry ice
Growth medium: For recommended growth and recombinant retrovirus production conditions, see Morgenstern & Land. 1990. Nucleic Acids Research. 18:3587-96. PMID: 2194165
References:

Littlewood et al. 1995. Nucleic Acids Res. 23(10):1686-90. PMID: 7784172.

A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins.

Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.

Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.

Related Tools for Cell lines

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162284 pBABE-hygro Vector Key Info View Tool
162285 pBABE-zeo Vector Key Info View Tool
151470 pBABE-puro Vector Key Info View Tool
162286 pBABE-neo Vector Key Info View Tool

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