HCT 116 p300 KO [D10] Cell Line

HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular, adhesion and migration

Contributors

Inventor Institute
Carlos Caldas Cancer Research UK Cambridge Institute
Cat. #: 151479
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer;Cell biology;Drug development
Organism: Human
Tissue: Colon
Model: Knock-Out
Growth properties: Adhesion properties; invasion; migration
Cancer Types In Detail: Carcinoma
Product description: The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT116), p300 KO Clone D10 (HCT116), p300 KO Clone F5 (HCT116), p300 KO Clone F2 (HCT116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The best 2 KO clones are D10 and F5. Anyone requesting the cell line should use the parental cell line that the KOs were generated from. The p300 KO lines demonstrate an aggressive cancer phenotype in vitro, with loss of cell-cell adhesion, defects in cell-matrix adhesion, and increased migration through collagen matrices. p300 is a transcriptional cofactor involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties. Somatic inactivating mutations of p300 are associated with several cancers including breast, colorectal and gastric cancers. CBP was not manipulated in this cell line.
Additional notes: CBP was not manipulated in this cell line
Cellosaurus ID: CVCL_HG27
Parental cell line: HCT 116
Disease: Cancer
Format: Frozen
Storage conditions: -20° C
Shipping conditions: Dry ice
Growth medium: McCoy's 5A medium + 10% FCS
Recommended controls: HCT 116 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.

?-HPV 5 and 8 E6 disrupt homology dependent double strand break repair by attenuating BRCA1 and BRCA2 expression and foci formation.

Iyer et al. 2007. Oncogene. 26(1):21-9. PMID: 16878158.

Bundy et al. 2006. Cancer Res. 66(15):7606-14. PMID: 16885360.

Metabolic consequences of p300 gene deletion in human colon cancer cells.

p300 is required for orderly G1/S transition in human cancer cells.

Krubasik et al. 2006. Br …

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Adapted from Wallace et al. 2015. PLoS Pathog. 11(3)

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