C-Raf KI D486A Cell Line

The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling.

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151631
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell signaling and signal transduction;Drug development
Organism: Mouse
Tissue: Embryo
Model: Knock-In
Product description: The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling. See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
Production details: Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture.
Parental cell line: See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from.
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FCS, 100U/mol penicillin/streptomycin
References:

Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.

CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.

Images

View Gallery
C-Raf KI D486A Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Related Tools for Cell lines

Cat. # Tool Name
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151559 MEF UNG KO Cell Line Key Info View Tool
151446 FLYRD18 Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151655 FLYA-13 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.