143B m.8993T>G mutant mTUNE mT10 cell line
These lines are derivatives of the m.8993T cybrid cell developed by Prof Eric Schon, and are of of 143B human osteosarcoma lineage.
Contributors
| Inventor | Institute |
|---|---|
| Payam Gammage | Cancer Research UK, Glasgow: The Beatson Institute |
| Cat. #: | 160453 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Bone cancer |
| Research Fields: | Cancer;Metabolism |
| Organism: | Human |
| Tissue: | Bone |
| Model: | Tumour line |
| Cancer Types In Detail: | Bone;Osteosarcoma |
| Alternate name: | mTUNE 1, mT1 |
|---|---|
| Product description: | The cellular metabolic environemnt is influenced by relative levels of coexistant mutant and wild-type mitochondrial DNAs (heteorplasmy) in a range of diseases for which mitochondrial dysfunction is a feature. Disease phenotypes may be rescued by returning heteroplasmy towards wild-type levels. To investigate this approach, the team led by Payam Gammage have devised a series of cell lines which stably express stable levels of mutatnt m.8993T>G mtDNA heteroplasmy. Cell lines bearing m.8993T>G heteroplasmy of 70 and 10% are available at CancerTools.org. These lines are derivatives of the m.8993T cybrid cell developed by Prof Eric Schon, and are of of 143B human osteosarcoma lineage. This is part of a series of two cell line (mTUNE); see Related research tools tab. |
| Production details: | mTUNE cells were generated by Dr Michal MinczukÄË?Â?Âs lab and derive from female human osteosarcoma 143B (RRID: CVCL_2270) cybrid cells (Porteous et al., 1998), after correction of m.8993T>G mutation with mitochondrially-targeted zinc finger nucleases (Gammage et al., 2016a). See Gaude et al., 2018 for further details. |
| Additional notes: | This is part of a series of three cell line (mTUNE); see Related Reagents tab. |
| Parental cell line: | 143B |
| Disease: | Cancer |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | high glucose DMEM media supplemented with L-glutamine and 10S and pen/strep |
| Subculture routine: | Grow in regular dishes or flasks (10cm dish or 75cm2 flask). Passage 2 or 3 times a week, split when confluent (wash with PBS and then use trypsin to split). There is no maximum passage number. 1:10 for 3 splits a week, 1:20 Friday to Monday |
| References: |
Gammage et al. 2018. Nat Med. 24(11):1691-1695. PMID: 30250142. Gammage et al. 2018. Methods Mol Biol. 1867:43-56. PMID: 30155814. Gaude et al. 2018. Mol Cell. 69(4):581-593.e7. PMID: 29452638. Gammage et al. 2016. Methods Mol Biol. 1351:145-62. PMID: 26530680. Gammage et al. 2016. Nucleic Acids Res. 44(16):7804-16. PMID: 27466392. Gammage et al. 2014. EMBO Mol Med. 6(4):458-66. PMID: 24567072. |
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