RalGDS Floxed Mouse

The knock-out mouse was developed by Chris Marshall at the ICR and used to demonstrate a significant role for RalGDS in Ras-dependent carcinogenesis in vivo.

Contributors

Inventor Institute
Chris Marshall The Institute of Cancer Research
Cat. #: 153630
Research Fields: Cancer;Cell signaling and signal transduction;Genetics
Target: RalGDS
disease: Melanoma
Model: Conditional KO
Zygosity: Homozygous
Alternate name: Friend Virus B, Ral Guanine Nucleotide Dissociation Stimulator, Ral Guanine Nucleotide Exchange Factor, RalGEF, RGF, KIAA138, RalGDS, RGDS
Product description: The knock-out mouse was developed by Chris Marshall at the ICR and used to demonstrate a significant role for RalGDS in Ras-dependent carcinogenesis in vivo. Lack of RalGDS resulted in reduced tumour incidence, size and progression to malignancy in multistage skin carcinogenesis, and reduced transformation by Ras in tissue culture. Experiments performed in cells isolated from skin tumours suggested that RalGDS mediates cell survival through the activation of the JNK/SAPK pathway. Mouse deficient in RalGDS (a member of the RalGEF family, which control the activity of the small GTPases RalA and RalB, and also have Ras binding domains).
Conditional: Yes
Genetic background: Mouse RalGDS genomic clones were obtained by screening a 129/SvJ BAC library. A targeting vector was designed using a DNA fragment extending from intron 7 to the 3'UTR, cloned into the pKO scrambler 901 vector. A neomycin cassette flanked by two loxP sites was cloned upstream of exon 16 and a 3rd loxP site was located in exon 8. The exons flanked by loxP sites comprise part of the catalytic domain of RalGDS and residues involved in the binding of the exchange factor to Ras. The construct was electroporated into RW4 ES cells and clones positive for recombination were then transiently transfected with pcrePac vector to eliminate the neomycin cassette. Cells carrying the RalGDS allele lacking exons 9-15 were injected into MF-1 blastocysts and germline-transmitting chimeric mice were obtained. As a result of Cre-mediate recombination, a RasGDS floxed allele in which loxP sites flank the exon 9-15 region was obtained.
Production details: Mouse RalGDS genomic clones were obtained by screening a 129/SvJ BAC library. A targeting vector was designed using a DNA fragment extending from intron 7 to the 3'UTR, cloned into the pKO scrambler 901 vector. A neomycin cassette flanked by two loxP sites was cloned upstream of exon 16 and a 3rd loxP site was located in exon 8. The exons flanked by loxP sites comprise part of the catalytic domain of RalGDS and residues involved in the binding of the exchange factor to Ras. The construct was electroporated into RW4 ES cells and clones positive for recombination were then transiently transfected with pcrePac vector to eliminate the neomycin cassette. Cells carrying the RalGDS allele lacking exons 9-15 were injected into MF-1 blastocysts and germline-transmitting chimeric mice were obtained. As a result of Cre-mediate recombination, a RasGDS floxed allele in which loxP sites flank the exon 9-15 region was obtained.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Gonzlez-Garca et al. 2005. Cancer Cell. 7(3):219-26. PMID: 15766660.

RalGDS is required for tumor formation in a model of skin carcinogenesis.

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