LifeAct-GFP Mouse

This Cre-inducible transgenic mouse can conditionally express cell-type specific LifeAct-GFP

Contributors

Inventor Institute
Laura Machesky Cancer Research UK, Glasgow: The Beatson Institute
Cat. #: 153317
Research Fields: Cell biology;Genetics
Target: Actin cytoskeleton
Model: Reporter
Zygosity: Heterozygous
Strain: C57BL/6
Alternate name: Hypoxanthine Phosphoribosyltransferase 1, HGPRTase, HGPRT, HPRT, F-actin
Product description: This Cre-inducible transgenic mouse can conditionally express cell-type specific LifeAct-GFP. This is possible due to a floxed neomycin resistance 'STOP' cassette encoded upstream of the LifeAct-GFP fusion cDNA in the Hprt locus of the mouse X chromosome. LifeAct is a 17 amino acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics in vitro or in vivo. This enables live imaging of the actin cytoskeleton and therefore the study of many fundamental biological processes like cytokinesis. Incorporation of LifeAct in this specific and selective manner allows the study of actin dynamics in cell types, particularly primary cells, which are not readily transfectable. It also permits the study of intracellular actin in cells whilst present in their native environment. The ability to visualise actin in this way surpasses other reagents used to visualize the actin cytoskeleton like fluorescent phalloidin and anti-actin antibodies.
Conditional: Yes
Conditional description: Expression of Cre recombinase excises the lox stop lox cassette encoded upstream of the LifeAct-GFP marker enabling its expression.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Schachtner et al. 2012. Eur J Cell Biol. 91(11-12):923-9. PMID: 22658956.

Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.

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