Cat. #162157
Zc3h13-AID cell line
Cat. #: 162157
Sub-type: Primary
Organism: Mouse
Tissue: Blastocyst
Model: Genetically modified cell line; Knock in
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Institut Curie
Primary Citation: Tomasz Chelmicki et. al. 2021 Nature. 591(7849):312-316. PMID: 33442060
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Zc3h13-AID cell line
- Research fields: Stem cell biology
- Tool sub type: Primary
- Parental cell: E14 murine ES cells (129Ola strain)
- Organism: Mouse
- Gender: Male
- Tissue: Blastocyst
- Growth properties: Adherent
- Model: Genetically modified cell line; Knock in
- Model description: The cell line contains OsTIR1 at the TIGRE locus. The AID tag is inserted in the N terminus of the Zc3h13 gene.
- Crispr: Yes
- Conditional description: Depletion is achieved upon Auxin treatment (degradation of the AID-tagged proteins)
- Description: Murine embryonic stem cells with an inducible and reversible (via degron AID) KO of Zc3h13 - protein used for m6A methylation of RNA
Handling
- Format: Frozen
- Passage number: P5
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
- Cultured in antibiotics: Yes
- Characterisation tests: Western blotting to confirm the depletion of the protein
References
- Tomasz Chelmicki et. al. 2021 Nature. 591(7849):312-316. PMID: 33442060
