US/VOT-E36 Cell Line
Epithelial progenitors with potential to differentiate into sensory and nonsensory epithelial cells
Contributors
| Inventor | Institute |
|---|---|
| Matthew C Holley | University of Sheffield |
| Cat. #: | 153625 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Research Fields: | Developmental biology;Drug development;Genetics |
| Organism: | Mouse |
| Tissue: | Embryonic |
| Model: | Transgenic |
| Morphology: | Epithelial |
| Growth properties: | Adherent |
| Primary citation: | Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550 |
| Alternate name: | US/VOT-E36; Ventral Otocyst-Epithelial cell line number 36; VOT-E36; University of Sheffield/Ventral OTocyst-Epithelial 36 |
|---|---|
| Product description: | US/VOT-E36 cells represent epithelial progenitors with potential to differentiate into sensory and nonsensory epithelial cells. The conditionally immortal cell line was established from the ventral otocyst of the Immortomouse at embryonic day 10.5 (plug in mouse designated E0.5 and birth at E18-19). At this stage the sensory epithelia have not differentiated, and the epithelium is competent to form most of the cells within the cochlear duct, including primary sensory neurons. VOT-E36 cell… |
| CRISPR: | No |
| Conditional: | Yes |
| Conditional description: | The cells were derived from ImmortomiceTM (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100) carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the ?interferon-sensitive MHC Class 1 promoter. The cells proliferating culturing conditions: 33°C with ?IFN; and the cells differentiating culturing conditions: 39… |
| Production details: | Homozygous male Immortomice (originally derived from injected oocytes of CBA/Ca x C57BL/10 mice) were time-mated with wild-type C57Bl/6 female mice to produce heterozygous offspring. Animals were killed by cervical dislocation, in accordance with UK Home Office regulations. Otocysts were removed from E10 embryos under sterile conditions and then dissected to isolate the ventral region. Further selection of ventral otocyst explants was based on expression of the immortalizing gene (condition… |
| Cellosaurus ID: | CVCL_VC36 |
| Model description: | Conditionally immortalized cell line |
| disease: | Deafness |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | MEM with 10% FCS, 50 Units/ml y-IFN, L-glutamine |
| Temperature: | 33° C |
| Storage medium: | Cytiva HyCloneTM FetalClone II Serum (U.S.) Thermo Fisher |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Holley et al. 2007. Hear Res. 227(1-2):32-40. PMID: 16797894 Helyer et al. 2007. Eur J Neurosci. 25(4):957-73. PMID: 17331193 Liu et al. 2006. Otol Neurotol. 27(3):414-21. PMID: 16639283 Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550 |
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