Cat. #160443
UM-UC-14 (Human transitional cell carcinoma of the renal pelvis) cell line
Cat. #: 160443
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Bladder
Disease: Cancer
Model: Tumourigenic cell line
£575.00
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Contributor
Inventor: H. Grossman ; Anita Sabichi
Institute: University of Michigan
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: UM-UC-14 (Human transitional cell carcinoma of the renal pelvis) cell line
- Alternate name: UM-UC-14; UMUC-14; UM-UC14; UMUC14; University of Michigan-Urothelial Carcinoma-14
- Cancer: Genitourinary cancer
- Cancers detailed: Human bladder transitional cell carcinoma
- Research fields: Cancer
- Organism: Human
- Tissue: Bladder
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumourigenic cell line
- Conditional: Yes
- Description: UM-UC-14 are a human transitional cell carcinoma of the renal pelvis. These cells were assessed for their susceptibility to adenoviral mediated gene delivery, tumour growth in nude mice and differences in genetic alterations. Tumorigenicity studies in nude mice revealed the UM-UC-14 produced tumours, 1-1.5 cm in diameter in < 3 weeks. This cell line demonstrated efficient gene transduction via an adenoviral vector when compared to several of the other cells evaluated. UM-UC-14 also demonstrated one of the highest levels of Coxsackie adenovirus receptor expression of the cells tested. These assays allowed for characterization of some of the most important features in each of the respective lines in an effort to more accurately establish commonly observed phenomena across cells of the same type of neoplasm.
Handling
- Format: Frozen
- Growth medium: Culture medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). Culture conditions: 37°C with 5% CO₂ Sub-culture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂ 37°C.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: UM-UC-1 (Human bladder transitional cell carcinoma) cell line ; UM-UC-3 (Human bladder transitional cell carcinoma) cell line ; UM-UC-4 (Human adenocarcinoma lymph node metastasis) cell line ; UM-UC-5 (Human squamous cell carcinoma of the bladder) cell line ; UM-UC-7 (Human transitional cell carcinoma of the bladder) cell line ; UM-UC-9 (Human transitional cell carcinoma of the bladder) cell line ; UM-UC-10 (Human transitional cell carcinoma of the bladder) cell line
References
- Sabichi et al. 2006. J Urol. 175(3 Pt 1):1133-7. PMID: 16469639.
- Grossman et al. 1984. J Urol. 132(4):834-7. PMID: 6471236.
