T47D/TR-1 Cell Line

The T47D/S2 Cell line is the control sensitive line for the tamoxifen resistant T47D/TR-1 and T47D/TR-2 lines.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society
Cat. #: 152108
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Model: Cancer cell line
Growth properties: Adherent
Cancer Types In Detail: Breast carcinoma;Tamoxifen resistant
Primary citation: Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
Product description: The T47D/TR-1 cell line is a tamoxifen-resistant derivative of the T47D human breast cancer cell line. It was developed by adapting T47D/S2 cells to 4-hydroxytamoxifen under reduced serum conditions to induce tamoxifen resistance.
Conditional: Yes
Production details: Human breast cancer cell line derived from T47D/S2 cells by long-term culture in the presence of 4-hydroxytamoxifen under reduced serum conditions to induce tamoxifen resistance.
Cellosaurus ID: CVCL_1D36
Parental cell line: T47D/S2
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml + 1 uM tamoxifen. Fetal Calf Serum (FCS) typically contains less estrogen than Fetal Bovine Serum (FBS) and is the preferred supplement for this cell line.
Subculture routine: Following thawing and counting, dilute the cell suspension with sufficient medium and distribute 5 mL each into T25 flasks to achieve a seeding density of 1.8 – 2.0 x 10^4 / cm2. Place in 37°C, 5% CO₂ incubator. Change medium after 24 hours to remove residual DMSO and then every 2-3 days. Subculture routine: Split 1:7 weekly (slow growing cell line) with Trypsin-EDTA for detachment at 37 °C for 5 minutes. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: 10% DMSO in FCS
Mycoplasma free: Yes
Biosafety level: 1
References:

Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.

Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.

Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.

Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.

Images

View Gallery
Representative pictures of sensitive parental (T47D/S2 and T47D/S5); tamoxifen (TR-1 and TR-1) and fulvestrant (182R-1 and 182R-2) resistant cell lines treated for five days with dasatinib or DMSO (control). Adapted from Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.

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