#153169

T47D-E2J Cell Line

Please Enter Your Email ID
Email ID

Cat. #153169

T47D-E2J Cell Line

Cat. #: 153169

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Breast

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Joy Burchell ; Joyce Taylor-Papadimitriou

Institute: King's College London

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: T47D-E2J Cell Line
  • Alternate name: C2GnTC2GnT-L
  • Cancer: Breast cancer
  • Cancers detailed: Breast cancer
  • Research fields: Cancer;Drug development
  • Tool sub type: Continuous
  • Parental cell: T47D
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Conditional: No
  • Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-E2J, was created as a breast cancer model with an O-linked glycosylation profile similar to normal mammary epithelial cells. T47D-E2J cells overexpress the enzyme C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein.C2GnT1 is the dominant enzyme expressed in normal breast tissue known to convert core 1 glycoproteins to core 2 glycoproteins, by the addition of N-acetylglucosamine to N-acetylgalactosamine. Expression of the C2GnT1 enzyme has been found to be decreased or absent in most breast cancer cell lines despite still expressing mRNAs coding for this enzyme. Together with wild-type T47D and T47D-STn these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.

Target Details

  • Target: Glycosyltransferase - C2GnT1

Handling

  • Format: Frozen
  • Growth medium: DMEM 10% FCS and 500?„?žg/ml G418
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: T47D-STn Cell Line

References

  • Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
  • The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
  • Dalziel et al. 2001. J Biol Chem. 276(14):11007-15. PMID: 11118434.
  • The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1.