SK-N-SH Cell Line

SK-N-SH is a neuroblastoma cell line that displays epithelial morphology and grows in adherent culture.

Contributors

Inventor Institute
June L. Biedler, Barbara A. Spengler Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161937
Cancer types: Neurological cancer
Tissue: Brain
Gender: Female
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Neuroblastoma
Primary citation: Biedler et al. Cancer research. 33(11):2643-2652. PMID: 4748425.
Product description: SK-N-SH is a neuroblastoma cell line that displays epithelial morphology and grows in adherent culture.
Gender: Female
Products or characteristics of interest: Karyotype: The cell line is hyperdiploid human female (XX), with the modal chromosome number of 47. Normal chromosomes N9 and N22 are single. One copy of each of these chromosomes is structurally altered to form the two marker chromosomes 9q+ and 22q+., Chromosomes N7 is trisomic. Extra bands were found on one copy of chromosome N7, thereby forming a marker chromosome as described by R.C. Seeger. May have been translocated in part(s) to the q arms of chromosomes N9 and N22. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 2 PGM1, 1 PGM3, 1
Shipping conditions: Dry Ice
Growth medium: 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% DMSO
Biosafety level: 1
References:

Helson et al. 1975. Cancer Res. 35(9):2594-2599. PMID: 167965.

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