Cat. #161937
SK-N-SH Cell Line
Cat. #: 161937
Tissue: Brain
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: June L. Biedler, Barbara A. Spengler
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Biedler et al. Cancer research. 33(11):2643-2652. PMID: 4748425.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-N-SH Cell Line
- Cancer: Neurological cancer
- Cancers detailed: Neuroblastoma
- Gender: Female
- Tissue: Brain
- Donor: This cell line was established in 1970 from metastatic cells found in the bone marrow aspirate of a four-year-old female of unknown ethnicity.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: The cell line is hyperdiploid human female (XX), with the modal chromosome number of 47. Normal chromosomes N9 and N22 are single. One copy of each of these chromosomes is structurally altered to form the two marker chromosomes 9q+ and 22q+., Chromosomes N7 is trisomic. Extra bands were found on one copy of chromosome N7, thereby forming a marker chromosome as described by R.C. Seeger. May have been translocated in part(s) to the q arms of chromosomes N9 and N22. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 2 PGM1, 1 PGM3, 1
- Description: SK-N-SH is a neuroblastoma cell line that displays epithelial morphology and grows in adherent culture.
- Application: 3D cell culture, Immunology, Neuroscience
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type A; Rh+
Applications
- Application: 3D cell culture, Immunology, Neuroscience
Handling
- Growth medium: 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: XCSF1PO: 11D13S317: 11D16S539: 8,13D5S818: 12D7S820: 7,10TH01: 7,10TPOX: 8,11vWA: 14,18D3S1358: 15,16D21S11: 31,31.2D18S51: 13,16Penta_D: 10,12Penta_E: 7,11FGA: 23.2,24D8S1179: 15D19S433: 13,14D2S1338: 17,19
References
- Helson et al. 1975. Cancer Res. 35(9):2594-2599. PMID: 167965.
