SK-N-BE(2) Cell Line

SK-N-BE(2) is a neuroblastoma cell line that displays MYCN amplification. These cells have moderate dopamine-b-hydroxylase activity and low-choline acetyltransferase activity. The SK-N-BE(2) cells are known to form tumours in immunocompromised mice.

Contributors

Inventor Institute
June L. Biedler Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161921
Cancer types: Neuroblastoma
Organism: Human
Tissue: Brain
Gender: Male
Model: Tumourigenic
Morphology: Neuroblast
Growth properties: Mixed: adherent and suspension
Cancer Types In Detail: Neuroblastoma
Primary citation: Biedler et al. 1976. Journal of the National Cancer Institute. 57: 683-695. PMID: 62055.
Product description: SK-N-BE(2) is a neuroblastoma cell line that displays MYCN amplification. These cells have moderate dopamine-b-hydroxylase activity and low-choline acetyltransferase activity. The SK-N-BE(2) cells are known to form tumours in immunocompromised mice.
Gender: Male
Additional notes: The cells exhibit moderate levels of dopamine beta hydroxylase activity. SK-N-BE(2) cells have a reported saturation density greater than 1 X 106 cells/cm2. The morphology of the cells varies with some cells having long processes and others that are epithelioid like. The cells will aggregate, form clumps and float.
Model description: Tumourigenic; an inoculum of 107 cells produced tumours in cortisonised hamster cheek pouches with 18% frequency
Products or characteristics of interest: Karyotype: modal number = 44; one or more HSR bearing chromosomes
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: 1:1 mixture of EMEM:F12 Medium, then supplemented with FBS to a final concentration of 10%
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Barnes et al. 1981. In Vitro. 17: 619-131. PMID: 7327593.

Biedler et al. 1976. Science. 191: 185-187. PMID: 942798.

Biedler et al. 1978. Cancer Research. 38: 3751-3757. PMID: 29704.

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