SH-SY5Y Cell Line

SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line.

Contributors

Inventor Institute
June L. Biedler, Barbara A. Spengler Memorial Sloan-Kettering Cancer (MSK)
Cat. #: 161932
Cancer types: Neurological cancer
Tissue: Bone, Marrow tissue
Gender: Female
Morphology: Epithelial
Growth properties: Mixed: adherent and suspension
Cancer Types In Detail: Neuroblastoma
Primary citation: Ross et al. 1983. Journal of the National Cancer Institute. 71(4):741-7. PMID: 6137586.
Product description: SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line.
Gender: Female
Additional notes: SH-SY5Y cells have a reported saturation density greater than 1 X 106 cells/cm2. They are reported to exhibit moderate levels of dopamine beta hydroxylase activity.
Products or characteristics of interest: Karyotype: Modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q
Shipping conditions: Dry Ice
Growth medium: 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% DMSO
Biosafety level: 1
References:

Zanker et al. 2023. Frontiers in molecular neuroscience. 16:1280556. PMID: 38098942.

Thirant et al. 2023. Nature communications. 14(1):2575. PMID: 37142597.

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