SARS-CoV-2 RBD fragment cell line

The SAR-CoV-2 RBD fragment expressed by this cell line has been successfully used to make ELISA assays for detection of coronavirus infection in human patients.

Contributors

Institute
The University of Minnesota, Twin Cities
Cat. #: 160855
Research Fields: Biochemistry;Drug development;Immunology;Microbiology
Organism: Human
Cancer Types In Detail: Antigen;Protein fragment;Receptor binding domain
Alternate name: Recombinant SARS-CoV-2 RBD
Product description: The SAR-CoV-2 RBD fragment expressed by this cell line has been successfully used to make ELISA assays for detection of coronavirus infection in human patients.
Production details: To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
Parental cell line: HEK293F
disease: Coronavirus
Growth medium: The cells are cultured in FreeStyle 293 Expression Medium from Thermo Fisher.
Recommended controls: No
References:

Shang et al. 2020. Nature. 581(7807):221-224. PMID: 32225175.

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