RMA.Trh4 Db KO cells

This cell line overexpresses the ER-resident ceramide synthase Trh4 (transduced by CRISPR) and lacks the H2-Db gene. It serves as a control in helping to understanding T-cell recognition of the Trh4-derived peptide presented by the MHC class I molecule H2-Db.

Contributors

Inventor Institute
Thorbald Van Hall Leiden University and Leiden University Medical Center; Stichting Oncode Institute
Cat. #: 157677
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Cancer
Organism: Mouse
Tissue: Lymphatic Tissue
Model: Knock-Out
Cancer Types In Detail: Mouse Leukemia
Product description: This cell line overexpresses the ER-resident ceramide synthase Trh4 (transduced by CRISPR) and lacks the H2-Db gene. It serves as a control in helping to understanding T-cell recognition of the Trh4-derived peptide presented by the MHC class I molecule H2-Db. This peptide-epitope is a prototypic example of a neo-antigen selectively presented by cells with processing defects in the classical MHC class I (MHC-I) pathway. RMA cells have an intact processing pathway and a functional TAP peptide transporter, but overexpress the Trh4 protein and therefore could present the Trh4 peptide. However, no T cell recognition can be observed due to the absence of the MHC class I H2-Db.This population acts as a clear control. CRISPR edited RMA cells.
Production details: Retroviral transduction of the mouse Trh4 gene in an IRES-GFP construct and CRISPR/Cas9 technology
Additional notes: CRISPR edited RMA cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide…
Cellosaurus ID: CVCL_J385
Parental cell line: RMA
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Suspension cells in DMEM+8% FCS
Recommended controls: RMA.Trh4 Kb KO cells
Mycoplasma free: Yes
Biosafety level: 1
References:

Doorduijn et al. 2018. Oncoimmunology. 7(3):e1382793. PMID: 29399388.

Related Tools for Cell lines

Cat. # Tool Name
151014 MCF7 AREc32 Cell Line Key Info View Tool
151450 JIM3 Cell Line Key Info View Tool
151559 MEF UNG KO Cell Line Key Info View Tool
151663 MEF PKCe KO KI Cell Line Key Info View Tool
151665 MEF PKCe KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.