#

RCC-FG2 + PBRM1 Clone 13

RCC-FG2 + PBRM1 Clone 13

Availability: 3-4 weeks

Organism: Human

Tissue: kidney

Model: Genetically modified

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jessica Downs, Hugang Feng

Institute: The Institute of Cancer Research

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: RCC-FG2 + PBRM1 Clone 13
  • Parental cell: RCC-FG2
  • Clone: 13
  • Organism: Human
  • Gender: Male
  • Tissue: kidney
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Genetically modified
  • Model description: Conditional re-expression of wild type PBRM1 gene
  • Crispr: Yes
  • Conditional: Yes
  • Conditional description: Conditional expression of wild type PBRM1 gene following deoxycycline treatment
  • Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
  • Description: RCC-FG2 cell line deficient for PBRM1 stably expressing DOX-inducible PBRM1 construct (Clone 13). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
  • Application: Chromatin remodelling biology studies
  • Biosafety level: 1
  • Cellosaurus id: CVCL_5873

Applications

  • Application: Chromatin remodelling biology studies

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 + 10%FBS + 1% Penicillin/Streptomycin
  • Temperature: 37C
  • Atmosphere: 5% CO2 in air
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: 1% Penicillin/Streptomycin
  • Mycoplasma free: Yes
  • Str profiling: See Cellosaurus profile of parental cell line

References

  • Unpublished