Cat. #162178
POLE c.1373A?>?T expressing HEK293T cell line (isogenic)
Cat. #: 162178
Organism: Human
Tissue: Kidney
Model: Genetically modified cell line; knock-in
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Marit Otterlei
Institute: Norwegian University of Science and Technology
Primary Citation: Rocque et al. (2023). Mol Genet Genomics. 298(3):555-566, PMID: 36856825
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: POLE c.1373A?>?T expressing HEK293T cell line (isogenic)
- Parental cell: HEK293T
- Organism: Human
- Gender: Female
- Tissue: Kidney
- Donor: Foetus
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Genetically modified cell line; knock-in
- Model description: c.1373A > T POLE variant gene expression
- Crispr: Yes
- Description: HEK293T overexpressing the c.1373A > T POLE variant
- Application: Cell culture, SupF mutagenesis assay
Target Details
- Target: POLE (DNA Polymerase Epsilon, Catalytic Subunit)
- Target background: The POLE encodes the catalytic subunit of the DNA polymerase epsilon, which is involved in DNA repair. Mutations in the POLE gene are associated with predisposition towards colorectal adenomas and carcinomas.
Applications
- Application: Cell culture, SupF mutagenesis assay
Handling
- Growth medium: Dulbecco's Modified Eagle's Medium (DMEM), supplemented with 10% Fetal Bovine Serum (FBS)
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Characterisation tests: SupF mutagenesis assay
Related Tools
- Related tools: WT POLE overexpressing HEK293T cell line, POLE c.1373A > T(p.Tyr458Phe) overexpressing HEK293T cell line, POLE c.1270C > G(p.Leu424Val) overexpressing HEK293T cell line POLE c.1089C > A(p.Asn363Lys) overexpressing HEK293T cell line,
References
- Rocque et al. (2023). Mol Genet Genomics. 298(3):555-566, PMID: 36856825
