NHE-3 expressing PS120 cell line

An isoform of the Na+/H+ exchanger, NHE-3, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then […]

Contributors

Inventor Institute
Mark Donowitz Johns Hopkins University
Cat. #: 156414
Unit size: 1×10^6 cells / vial
Research Fields: Cell signaling and signal transduction;Drug development
Product description: An isoform of the Na+/H+ exchanger, NHE-3, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:â?‚€?‹Â˜ NHE-3 is essential in studies related to diarrhea and constipation
Parental cell line: CCL39
disease: Diarrhea and constipation
Format: Frozen
Shipping conditions: Dry ice

Related Tools for Cell lines

Cat. # Tool Name
151446 FLYRD18 Cell Line Key Info View Tool
151447 CMT 64 Cell Line Key Info View Tool
151480 HEK293A EGFP-LC3 Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151663 MEF PKCe KO KI Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.