Msh3-/- SVG-A Cell Line

The MSH3 gene encodes a DNA mismatch repair protein important in certain cancer types and in certain neurodegenerative diseases.

Contributors

Inventor Institute
Robert Lahue National University of Ireland Galway ; Brown University
Cat. #: 153562
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell biology;Genetics
Organism: Human
Tissue: Brain
Model: Knock-Out
Growth properties: Adherent cell line
Alternate name: DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, DUP, Mismatch repair protein 1, MRP1
Product description: The MSH3 gene encodes a DNA mismatch repair protein important in certain cancer types and in certain neurodegenerative diseases.Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies.While ~98% of Msh3 protein is lost in these cells, the abundance of the related proteins Msh2 and Msh6 appear unaffected. This is relevant to DNA mismatch repair.
Conditional: No
Production details: Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies.
Additional notes: CRISPR edited cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide the…
Parental cell line: SVG-A immortalized human astrocytes
Disease: Neurodegenerative diseases
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM supplemented with 10% FBS
Recommended controls: SVG-A Cell Line, SVG-A Msh3 1.7X Cell Line (derived from Msh3-/- SVG-A Cell Line)
Mycoplasma free: Yes
Biosafety level: 1
References:

Keogh et al. 2017. Nucleic Acids Res. 45(17):10068-10078. PMID: 28973443.

MutS? abundance and Msh3 ATP hydrolysis activity are important drivers of CTGCAG repeat expansions.

Images

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Western blot comparison of Msh3-/- SVG-A cell line and parent unmutated SVG-A cell line (Msh3+/+). While ~98% of Msh3 protein is lost in the Msh3-/- cells; the abundance of the related proteins Msh2 and Msh6 appear unaffected. This is relevant to DNA mismatch repair. Reference

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