#153562

Msh3-/- SVG-A Cell Line

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Cat. #153562

Msh3-/- SVG-A Cell Line

Cat. #: 153562

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Tissue: Brain

Disease: Neurodegenerative diseases

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Robert Lahue

Institute: National University of Ireland Galway ; Brown University

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Msh3-/- SVG-A Cell Line
  • Alternate name: DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, DUP, Mismatch repair protein 1, MRP1
  • Research fields: Cancer;Cell biology;Genetics
  • Tool sub type: Continuous
  • Parental cell: SVG-A immortalized human astrocytes
  • Organism: Human
  • Tissue: Brain
  • Disease: Neurodegenerative diseases
  • Growth properties: Adherent cell line
  • Model: Knock-Out
  • Conditional: No
  • Description: The MSH3 gene encodes a DNA mismatch repair protein important in certain cancer types and in certain neurodegenerative diseases.Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies.While ~98% of Msh3 protein is lost in these cells, the abundance of the related proteins Msh2 and Msh6 appear unaffected. This is relevant to DNA mismatch repair.
  • Application: Applications tested: DNA repair assays Microsatellite instability Trinucleotide expansion assay Cell background for rescue with wild type or variant Msh3 clones Immunoprecipitation control for Msh2-Msh3 protein complex Functional analysis of Msh3 variants
  • Production details: Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies.
  • Biosafety level: 1
  • Additional notes: CRISPR edited cells. Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide the...
  • Recommended controls: SVG-A Cell Line, SVG-A Msh3 1.7X Cell Line (derived from Msh3-/- SVG-A Cell Line)

Target Details

  • Target: Msh3

Applications

  • Application: Applications tested: DNA repair assays Microsatellite instability Trinucleotide expansion assay Cell background for rescue with wild type or variant Msh3 clones Immunoprecipitation control for Msh2-Msh3 protein complex Functional analysis of Msh3 variants
  • Application notes: Cancer Research Technology Limited (trading research tools as CancerTools.org) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here: https://www.cancertools.org/tool-faqs#hs_cos_wrapper_widget_1649861453796 This license from ERS Genomics Ltd allows CancerTools.org to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. CancerTools.org can provide these modified CRISPR-Cas9 cell lines to comp...

Handling

  • Format: Frozen
  • Growth medium: DMEM supplemented with 10% FBS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: SVG-A Cell Line ; SVG-A Msh3 1.7X Cell Line

References

  • Keogh et al. 2017. Nucleic Acids Res. 45(17):10068-10078. PMID: 28973443.
  • MutS? abundance and Msh3 ATP hydrolysis activity are important drivers of CTGCAG repeat expansions.