MM163 cell line
Unpigmented cell line derived from a skin metastasis. This cell line displays an invasive/Mesenchymal_like phenotype.
Contributors
| Inventor | Institute |
|---|---|
| Ghanem Elias, Ahmad Najem | Université libre de Bruxelles |
| Cat. #: | 162373 |
|---|---|
| Cancer types: | Melanoma |
| Organism: | Human |
| Tissue: | Skin |
| Gender: | Female |
| Model: | Tumour cell line |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Melanoma |
| Primary citation: | Wouters, J et al. Nat Cell Biol 2020. 22, 986–998.PMID: 32753671. |
| Product description: | Unpigmented cell line derived from a skin metastasis. This cell line displays an invasive/Mesenchymal_like phenotype. |
|---|---|
| Gender: | Female |
| CRISPR: | No |
| disease: | Melanoma |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry Ice |
| Growth medium: | Ham’s F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 µg/ml). Renewal 2 to 3 times per week |
| Subculture routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:5 |
| Temperature: | 37°C |
| Atmosphere: | 5% CO2 |
| Biosafety level: | 2 |
| References: |
Rambow et al. 2018. Cell 174, 843–855.e19. PMID: 30033366 Wouters et al. 2020. Nat Cell Biol 22, 986–998. PMID: 32747822 Rapino et al. 2018. Nature 558, 605–609. PMID: 29925948 |
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