Cat. #162028
Melan-pe1 cell line
Cat. #: 162028
Availability: 8-10 weeks
Organism: Mouse
Tissue: Skin
Disease: Hermansky-Pudlak syndrome
Model: Immortalised non-cancerous cell lines
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Elena Sviderskaya
Institute: St. George's University London
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Melan-pe1 cell line
- Organism: Mouse
- Tissue: Skin
- Disease: Hermansky-Pudlak syndrome
- Morphology: Small, oval-fusiform
- Model: Immortalised non-cancerous cell lines
- Description: The melan-pe1 line is an immortal melanocyte cell line derived from neonatal Ap3b1pe/Ap3b1pe C57BL/6J mice. These cell lines lack Ap3b1, a subunit of the adapter protein-3 (AP-3) complex. AP-3 is involved in sorting the key melanogenic enzyme tyrosinase from early-endosomes towards melanosomes. Pathogenic variants in the human ortholog of Ap3b1 cause a form of Hermansky-Pudlak syndrome (HPS), HPS-2. HPS is a group of disorders characterised by hypopigmentation, bleeding tendency and other variable symptoms. These cells are a useful model for HPS and for the study of trafficking of cargoes towards melanosomes.
- Application: Melanocyte cell biology
Applications
- Application: Melanocyte cell biology
Handling
- Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
- Storage conditions: liquid N2
