MEF ULK1/2 WT (SIM) Cell Line

MEF ULK1/2 WT (SIM) cell line can be used as a wild-type control with ULK1 and ULK2 KO MEF cell lines. Useful to study ULK1-and ULK-2 dependent processes

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151714
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Autophagy
Product description: The MEF ULK1/2 WT (SIM) cell line can be used as a wild-type control with ULK1 and ULK2 KO MEF cell lines and is useful for studying ULK1-and ULK-2 dependent processes, including autophagy. Mouse embryonic fibroblasts derived from a wild-type littermate to the ULK1 KO and immortalized by serial passaging (spontaneous transformation).
Conditional: Yes
Production details: Cells were derived from mouse embryos at E13.5. Embryos were decapitated and eviscerated and the remaining tissues were dissociated to obtain MEFs. This cell line is a primary cell line immortalized by SIM.
Cellosaurus ID: CVCL_5A50
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.

Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.

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