#151714

MEF ULK1/2 WT (SIM) Cell Line

Cat. #151714

MEF ULK1/2 WT (SIM) Cell Line

Cat. #: 151714

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Embryo

Disease: Cancer

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Sharon Tooze

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MEF ULK1/2 WT (SIM) Cell Line
  • Research fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer
  • Growth properties: Autophagy
  • Model: Knock-Out
  • Conditional: Yes
  • Description: The MEF ULK1/2 WT (SIM) cell line can be used as a wild-type control with ULK1 and ULK2 KO MEF cell lines and is useful for studying ULK1-and ULK-2 dependent processes, including autophagy. Mouse embryonic fibroblasts derived from a wild-type littermate to the ULK1 KO and immortalized by serial passaging (spontaneous transformation).
  • Production details: Cells were derived from mouse embryos at E13.5. Embryos were decapitated and eviscerated and the remaining tissues were dissociated to obtain MEFs. This cell line is a primary cell line immortalized by SIM.
  • Cellosaurus id: CVCL_5A50

Target Details

  • Target: ULK1

Handling

  • Format: Frozen
  • Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: MEF ULK1 KO (SIM) Cell Line ; MEF ULK2 KO (SIM) Cell Line ; MEF ULK1 ULK2 DKO (SIM) Cell Line ; MEF ULK1 ULK2 DKO (SV40) Cell Line ; MEF ULK1/2 WT(SV40) Cell Line ; MEF ULK1 KO (SV40) Cell Line ; MEF ULK2 KO (SV40) Cell Line

References

  • McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
  • Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
  • Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.
  • Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.