#154147

MDA-MB-231 D3H2LN-Luc TetOn-3G cell line

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Cat. #154147

MDA-MB-231 D3H2LN-Luc TetOn-3G cell line

Cat. #: 154147

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Reporter

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Heather Mckinnon ; Justin Bower ; Daniel Croft

Institute: Cancer Research UK, Glasgow: The Beatson Institute

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MDA-MB-231 D3H2LN-Luc TetOn-3G cell line
  • Cancer: Breast cancer
  • Cancers detailed: Triple negative breast (TNBC)
  • Research fields: Cancer;Cell biology;Drug development;Genetics
  • Tool sub type: Continuous
  • Parental cell: MDA-MB-231 D3H2LN-Luc
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Growth properties: Adherent cell line
  • Model: Reporter
  • Conditional: Yes
  • Conditional description: When a gene of interest is introduced under the control of the tetracycline (Tet)-regulated transactivator Tet-On 3G expression can be induced by administration of doxycycline. Luciferase expression is non-conditional.
  • Description: This cell line contains a tetracyline inducible promoter construct ready for cloning in a gene of interest. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Unbekandt et al., 2014 introduced their genes of interest, the kinases MRCK?, ROCK1 and ROCK2 under the tetracycline inducible promoter, to study the impact of their expression and inhibition on migration of the cells. Useful for the study of breast cancer cell cytoskeleton reorganisation, cell migration and the development of therapies that inhibit the ability of primary tumours to metastasize. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.
  • Production details: Generated an MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G
  • Biosafety level: 1
  • Additional notes: Tet-On System owned by TET Systems GmbH & Co. KG.
  • Recommended controls: HeLa TetOn-3G

Handling

  • Format: Frozen
  • Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.
  • A novel small-molecule MRCK inhibitor blocks cancer cell invasion.