MCF7/EGFP_LC3 – Mito DsRed

Breast cancer cell line MCF7 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor.

Contributors

Inventor Institute
T.R. Santhosh Kumar Rajiv Gandhi Centre for Biotechnology
Cat. #: 161159
Cancer types: Breast cancer
Research Fields: Cancer;Disease (not cancer);Metabolism
Organism: Human
Tissue: Breast
Gender: Female
Model: Transgenic
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Breast carcinoma
Primary citation: Lupitha SS, et.al. PMID: 32109534.
Product description: Breast cancer cell line MCF7 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor.
Gender: Female
CRISPR: No
Conditional: No
Parental cell line: MCF7
Disease: Cancer
Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or EMEM with 2mM L-Glutamine and 10% Fetal Bovine Serum.
Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm².
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: Growth medium + 10% DMSO
References:

Lupitha SS, et.al. PMID: 32109534.

Related Tools for Cell lines

Cat. # Tool Name
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
151579 U-2 OS Gal4-p300 Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool
151663 MEF PKCe KO KI Cell Line Key Info View Tool
151576 MEF RAF1 KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.