Leprosy T Cell Line

Leprosy is an ancient disease caused by gram positive, rod shaped bacilli called Mycobacterium leprae. Patients present with varied clinico-pathological disease depending on the host immune response to Mycobacterium leprae. Lepromatous (LL) patients represent with low T cell and high humoral immune response. The Th2 paradigm is thought to underlye lepromatous disease. Primary T-cells isolated […]

Contributors

Inventor
Pranab K Das
Cat. #: 154127
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Immunology
Organism: Human
Model: Primary line
Product description: Leprosy is an ancient disease caused by gram positive, rod shaped bacilli called Mycobacterium leprae. Patients present with varied clinico-pathological disease depending on the host immune response to Mycobacterium leprae. Lepromatous (LL) patients represent with low T cell and high humoral immune response. The Th2 paradigm is thought to underlye lepromatous disease. Primary T-cells isolated from skin biopsis can be used as a research tool.
Production details: T cell lines were generated from fresh skin biopsies. Biopsies were incubated in a 24 well plate coated with 10Â?„?žg/well fibronectin to facilitate spontaneous migration of T cells from the biopsy into the Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100Â?„?žg/ml streptomycin. After 5 days extravasated skin T cells were transferred to an uncoated 24 well plate and expanded by mitogenic stimulation with 0.05% PH…
Parental cell line: Skin of leprosy patient
disease: Leprosy
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100 ?„?žg/ml streptomycin plus mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.

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