Leprosy T Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Leprosy T Cell Line
• Research fields: Cell biology;Immunology
• Parental cell: Skin of leprosy patient
• Organism: Human
• Disease: Leprosy
• Model: Primary line
• Description: Leprosy is an ancient disease caused by gram positive, rod shaped bacilli called Mycobacterium leprae. Patients present with varied clinico-pathological disease depending on the host immune response to Mycobacterium leprae. Lepromatous (LL) patients represent with low T cell and high humoral immune response. The Th2 paradigm is thought to underlye lepromatous disease. Primary T-cells isolated from skin biopsis can be used as a research tool.
• Production details: T cell lines were generated from fresh skin biopsies. Biopsies were incubated in a 24 well plate coated with 10Â?„?žg/well fibronectin to facilitate spontaneous migration of T cells from the biopsy into the Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100Â?„?žg/ml streptomycin. After 5 days extravasated skin T cells were transferred to an uncoated 24 well plate and expanded by mitogenic stimulation with 0.05% PH...

Handling

• Format: Frozen
• Growth medium: Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100 ?„?žg/ml streptomycin plus mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice