#154127

Leprosy T Cell Line

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Cat. #154127

Leprosy T Cell Line

Cat. #: 154127

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Disease: Leprosy

Model: Primary line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Pranab K Das

Tool Details
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Leprosy T Cell Line
  • Research fields: Cell biology;Immunology
  • Parental cell: Skin of leprosy patient
  • Organism: Human
  • Disease: Leprosy
  • Model: Primary line
  • Description: Leprosy is an ancient disease caused by gram positive, rod shaped bacilli called Mycobacterium leprae. Patients present with varied clinico-pathological disease depending on the host immune response to Mycobacterium leprae. Lepromatous (LL) patients represent with low T cell and high humoral immune response. The Th2 paradigm is thought to underlye lepromatous disease. Primary T-cells isolated from skin biopsis can be used as a research tool.
  • Production details: T cell lines were generated from fresh skin biopsies. Biopsies were incubated in a 24 well plate coated with 10Â?„?žg/well fibronectin to facilitate spontaneous migration of T cells from the biopsy into the Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100Â?„?žg/ml streptomycin. After 5 days extravasated skin T cells were transferred to an uncoated 24 well plate and expanded by mitogenic stimulation with 0.05% PH...

Handling

  • Format: Frozen
  • Growth medium: Iscove's Modified Dulbecco Medium (IMDM) supplemented with 10% normal human serum, 1mM glutamine, 100U/ml penicillin and 100 ?„?žg/ml streptomycin plus mitogenic stimulation with 0.05% PHA in the presence of irradiated allogeniec feeder cells consisting of PBMCs from two unrelated donors EBV transformed B cells and 10U/ml recombinant human IL-2 for 10 days.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice