JU77R cell line

Contributors

Inventor Institute
Sarah A Martin Queen Mary University of London
Cat. #: 158349
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Lung cancer
Research Fields: Cancer
Organism: Human
Tissue: Lung
Model: Cancer Model
Growth properties: Human mesothelioma cell line resistant to pegylated arginine deiminase (ADI-PEG20).
Cancer Types In Detail: Lung
Production details: JU77 cells were incubated with 10ug/ml ADI-PEG20 in RPMI-1640 media supplemented with 2% FBS and re-treated every week. After approximately 2 months, colonies were picked and seeded in a 96-well plate with 10ug/ml ADI-PEG20. A surviving clone (JU777R) was gradually expanded and maintained in 1ug/ml ADI-PEG20.
Cellosaurus ID: TBC
Parental cell line: JU77
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI-1640 media (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, and 100ug/ml streptomycin at 37?‚°C/5% CO2.
References:

Locke et al. 2016. Cell Rep. 16(6):1604-1613. PMID: 27452468.

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