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FLYRD18 Cell Line
Exclusive toolA human retroviral packaging cell line used to produce high-titre, human serum-resistant recombinant retroviral vectors for gene transfer and gene therapy research.
Contributors
| Inventor | Institute |
|---|---|
| Mary Collins | The Institute of Cancer Research |
| SKU: | 151446 |
|---|---|
| Product description: | FLYRD18 is a human HT1080-derived retroviral packaging cell line developed for production of high-titre recombinant retroviral vectors. It produces helper-free vectors designed to resist inactivation by human serum/complement, addressing a key limitation of earlier murine packaging systems. FLYRD18 is suitable for gene transfer and gene therapy research applications where efficient retroviral vector production, human serum resistance and reduced risk of replication-competent retrovirus generation are important. |
| Conditional: | Yes |
| Production details: | Human; for details of production of FLYRD18 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248 |
| Cellosaurus ID: | CVCL_8871 |
| Parental cell line: | HT 1080 |
| Disease: | Cancer |
| Cat. #: | 151446 |
|---|---|
| Provenance and Ownership: | The FLYRD18 Cell Line is owned by Cancer Research UK. CancerTools supplies the FLYRD18 Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing FLYRD18 Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Blood cancer |
| Research Fields: | Genetics |
| Organism: | Human |
| Model: | Packaging |
| Growth properties: | Recombinant retroviral production |
| Cancer Types In Detail: | Fibrosarcoma |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | For recommended growth and recombinant retrovirus production conditions see Cosset F et al, Journal of Virology, 1995, v69 pp7430-7436 & Takeuchi Y et al, Journal of Virology, 1994, v68 pp8001-8007 |
| References: |
Gerin et al. 1999. Biotechnol Prog. 15(5):941-8. PMID: 10514266 Cosset et al. 1995. J Virol. 69(12):7430-6. PMID: 7494248. Takeuchi et al. 1994. J Virol. 68(12):8001-7. PMID: 7966590. |
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FLYRD18 Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC).
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