The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.
| Inventor | Institute |
|---|---|
| John Marshall | Queen Mary University of London |
| SKU: | 151626 |
|---|---|
| Product description: | The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides. |
| Production details: | The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturerÄË?Â?Âs instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a … |
| Cellosaurus ID: | CVCL_5F66 |
| Parental cell line: | A375P |
| Disease: | Cancer |
| Cat. #: | 151626 |
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| Provenance and Ownership: | The A375P beta6-puro Cell Line is owned by Cancer Research UK. CancerTools supplies the A375P beta6-puro Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing A375P beta6-puro Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Skin cancer |
| Research Fields: | Cancer;Cell biology;Cell signaling and signal transduction;Drug development |
| Organism: | Human |
| Model: | Transgenic |
| Growth properties: | A375P melanoma cells stably expressing integrin beta 6 |
| Cancer Types In Detail: | Melanoma |
| Format: | Frozen |
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| Storage conditions: | Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability. |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM + 10S/1.25ug/ml puromycin. Antibotic is not required all the time. |
| Recommended controls: | A375P parental line |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604. Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands. |
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