A375P beta6-puro Cell Line

The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.

Contributors

Inventor Institute
John Marshall Queen Mary University of London
SKU: 151626
Product description: The A375P beta6-puro Cell Line is a useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent A375 line. A375 melanoma cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6-binding peptides.
Production details: The human melanoma cell line A375P was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturerâ?‚€?‚™s instructions (Dynal; Invitrogen). The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a …
Cellosaurus ID: CVCL_5F66
Parental cell line: A375P
Disease: Cancer
Cat. #: 151626
Provenance and Ownership: The A375P beta6-puro Cell Line is owned by Cancer Research UK. CancerTools supplies the A375P beta6-puro Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing A375P beta6-puro Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Skin cancer
Research Fields: Cancer;Cell biology;Cell signaling and signal transduction;Drug development
Organism: Human
Model: Transgenic
Growth properties: A375P melanoma cells stably expressing integrin beta 6
Cancer Types In Detail: Melanoma
Format: Frozen
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry ice
Growth medium: DMEM + 10S/1.25ug/ml puromycin. Antibotic is not required all the time.
Recommended controls: A375P parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

DiCara et al. 2007. J Biol Chem. 282(13):9657-65. PMID: 17244604.

Structure-function analysis of Arg-Gly-Asp helix motifs in alpha v beta 6 integrin ligands.

Related Tools for Cell lines

Cat. # Tool Name
151448 CMT 167 Cell Line Key Info View Tool
151449 JIM1 Cell Line Key Info View Tool
151463 Omega-E Packaging Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.